CDK1-CYCLINB assay
Cyclin dependent kinase 1
Synonyms: CDC28A, CDC2
Family: CMGC
CDK1 is a highly conserved protein, which controls the cell cycle1. CDK1 becomes an active kinase in complex with cyclin B and plays an essential role in mitosis. Its activity increases at late G1, when cyclin concentrations rise, stays high until anaphase, when it drops because cyclins are destroyed. It was discovered that Cdk1 alone can drive progression of the mammalian cell cycle by complexing with all the phase specific cyclins in the absence of Cdk2, Cdk4, and Cdk62. RO-3306 – a selective and reversible inhibitor of the catalytic activity of human CDK1/cyclin B1 and CDK1/cyclin A complexes was previously identified3.
CDK1-CYCLINB kinase assay is run on mobility shift microfluidics platform (Caliper), which provides best in industry quality of data. We routinely run services associated with this kinase including: screening, profiling, dose-response studies and kinetic measurements. Please contact us for more information.
References:
1. Enserink JM, Kolodner RD. An overview of Cdk1-controlled targets and processes. Cell division. 2010 May 13;5(1):11.
2. Satyanarayana A, Kaldis P. A dual role of Cdk2 in DNA damage response. Cell division. 2009 May 18;4(1):9.
3. Vassilev LT, Tovar C, Chen S, Knezevic D, Zhao X, Sun H, Heimbrook DC, Chen L. Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proceedings of the National Academy of Sciences. 2006 Jul 11;103(28):10660-5.