CDK2-CYCLINE assay
Cyclin dependent kinase 2 (complex with cyclin E)
Synonyms: cell division protein kinase 2
Family: CMGC
CDK2 is a cyclin dependent protein kinase, which activity is restricted to the G1-S phase of the cell cycle. Originally it was thought that CDK2 is responsible for S phase initiation and progression, but it was shown that Cdk1 can take over Cdk2’s function in its absence in vivo1. However, Cdk2 is indefensible for proper DNA damage repair – it acts upstream in the DNA damage pathway and is involved in the activation of DNA repair proteins. It was also shown that the activation of CDK2/cyclin E is necessary for initiation of centrosome duplication (both separation of centrioles and procentriole formation)2.
CDK2-CYCLINE kinase assay is run on mobility shift microfluidics platform (Caliper), which provides best in industry quality of data. We routinely run services associated with this kinase including: screening, profiling, dose-response studies and kinetic measurements. Please contact us for more information.
References:
1. Satyanarayana A, Kaldis P. A dual role of Cdk2 in DNA damage response. Cell division. 2009 May 18;4(1):9.
2. Okuda M, Horn HF, Tarapore P, Tokuyama Y, Smulian AG, Chan PK, Knudsen ES, Hofmann IA, Snyder JD, Bove KE, Fukasawa K. Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication. Cell. 2000 Sep 29;103(1):127-40.