GCK assay

Germinal center kinase

Synonyms: MAP4K2, BL44, RAB8IP, “Mitogen-activated protein kinase kinase kinase kinase 2”

Family: STE

GCK kinase is a member of the germinal center kinases family of proteins that has been implicated in a wide variety of cellular processes including cell growth and proliferation, polarity, migration, and stress responses1. The biological functions of GCK are not well elucidated, it was shown to play role in regulating of NF-κB signaling contributing to cancer development for a subset of malignancies, and its pharmacological inhibition has been reported to reduce the viability of colon cancer cells2.

GCK kinase assay is run on mobility shift microfluidics platform (Caliper), which provides best in industry quality of data. We routinely run services associated with this kinase including: screening, profiling, dose-response studies and kinetic measurements. Please contact us for more information.


1. Schouest KR, Kurasawa Y, Furuta T, Hisamoto N, Matsumoto K, Schumacher JM. The germinal center kinase GCK-1 is a negative regulator of MAP kinase activation and apoptosis in the C. elegans germline. PLoS One. 2009 Oct 14;4(10):e7450.

2. Tan L, Nomanbhoy T, Gurbani D, Patricelli M, Hunter J, Geng J, Herhaus L, Zhang J, Pauls E, Ham Y, Choi HG. Discovery of type II inhibitors of TGFβ-activated kinase 1 (TAK1) and mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2). Journal of medicinal chemistry. 2014 Jul 30;58(1):183-96.