General Principle

Nanosyn provides in vitro kinase assays based on the microfluidic mobility shift platform of PerkinElmer (former Caliper). The method reports on the enzyme activity and takes the advantage of the shift in the electrophoretic mobility of a peptide substrate upon an enzymatic conversion.

The target enzyme is incubated with fluorescently labeled substrate and test compounds in a standardized reaction mixture in 384 well plates. Upon termination of the reaction, samples are introduced onto microfluidic chips. The samples migrate through channels in the chips and product and substrate are separated based on the difference in their charge and mass (electrophoretic mobility shift). Enzyme activity is measured by direct comparison of the fluorescence in the product and substrate peaks.

Detection of product and substrate in real time